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Enzymatic hollow fiber membrane bioreactor for penicilin hydrolysis
Wenten I.G.a, Widiasa I.N.a
a Department of Chemical Engineering, Institut Teknologi Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Continuous enzymatic reaction has been proven as an efficient technique for several industrial applications. In this study, a type of hollow fiber membrane bioreactor where penicillin acylase entrapped within membrane pores was applied to continuously hydrolyze Penicillin G. The influences of various operating conditions on immobilization and enzymatic reaction processes were assessed. A mathematical model of the reactor behaviour at steady state condition was also developed. The immobilization results show that penicillin acylase was entrapped more than 90% (100,000 u.a m-2). Due to the much smaller size of 6-APA compared to the membrane pore, the solute diffuses freely through the membrane. However, the immobilized enzyme membrane retained around 35% of the solute. In addition, Km of immobilized penicillin acylase (8.04 mM) was slightly higher than that of free penicillin acylase (7.75 mM). The theoretical results indicated that convective transport was the main mechanism of mass transport even in the case where flux was very low. Low flux rate is important to avoid gel formation or enzyme release from membrane pores and to maximize the degree of conversion.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Enzymatic reactions[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Hollow fiber membrane bioreactor,Penicillin acylase,Penicillin hydrolysis[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]Financial supporti s from theM inister of State for Researcha ndT echnologyR epublico f Indonesia under Grant No. 256/SP/RUT/BPPT/IV/1996. The supporto f X-Flow in thef orm of membranes is gratefully acknowledged. The authors are indebtedt o Budiraharjo, Soelistiono, Sylvia and Prima for technical assistance.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/S0011-9164(02)00789-0[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]