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Thermostable glucoamylase-type enzyme from Bacillus acidocaldarius RP1
Natalia D.a, Yuliani Y.a, Ermayadhie Y.a, Putra R.a, Sindumarta M.a
a Protein and Enzyme Research Group, Department of Chemistry, Institut Teknologi Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]We present an experiment that enables student to understand the properties of thermostable glucoamylase-type enzyme produced by thermophilic Bacillus acidocaldarius RP1 isolated from Cimanggu Hot Spring, West Java, Indonesia. In addition, the students also gain experience working with a thermostable microorganism and some techniques frequently used in biochemistry, e.g. ultrasonication, ammonium sulfate fractionation, and spectrophotometric analysis. The glucoamylase-type enzyme activities were detected both as cell-associated and in the culture supernatant. The 20-40% saturated ammonium sulfate fraction of glucoamylase-type enzyme exhibited an optimum temperature of 65 0C and an optimum pH of 4.5. The enzyme showed a Vmax of 600 milliunits/mg and Km of 11.7 mg/ml. © 2002 by The International Union of Biochemistry and Molecular Biology.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bacillus acidocaldarius,Glucoamylase-type enzyme,Thermostable enzyme[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1002/bmb.2002.494030060137[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]