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Culture-independent and culture-dependent approaches on microbial community analysis at gedongsongo (GS-2) hot spring
Aminin A.L.N.a, Warganegara F.M.a, Aditiawati P.a, Akhmalokaa
a Biochemistry Research Division, Institut Teknologi Bandung, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© IJIB, All rights reserved.Microbial community analysis was performed in one of Gedongsongo hot spring, namely GS-2, using culture-independent and -dependent approaches. Microbial samples were obtained from filtration and cultivation of hot spring water. Partial 16S rRNA gene sequences were amplified using a set of primers for highly variable V4-V5 regions. The amplicons were characterized using denaturing gradient gel electrophoresis (DGGE). The DGGE profile showed that there were three bands in each well with similar pattern, while the others bands appeared at various positions. Most of bands from the DGGE gels were excised, re-amplified, and directly sequenced. The comparison study of 340 bp from each band to another 16S ribosomal RNA sequences from database and the phylogenetic analysis revealed that the predominant bacteria were β-proteobacteria as revealed from filtration sample, while microorganisms from cultivation samples were mostly β-proteobacteria and Firmicutes. In addition there were two bands representing α-proteobacteria.The member of β-proteobacteria mostly come from family of Burkholderiaceae (Ralstonia or Burkholderia), while the other were Delftia (Commamodaceae family) and some uncultures β-proteobacteria. Most of Firmicutes member were belonged to Alicyclobacillus sp. Further analysis on the 16S rRNA gene sequences from member of Burkholderiaceae revealed that there was a unique deletion at position 559 (E. coli position) in all of the GS-2 samples. This deletion suggested that the microorganisms from Gedongsongo hot spring are unique strains.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]16S rRNA,DGGE,Simple enrichment,Thermophiles[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]