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Spectroscopic studies on the thermodynamic and thermal denaturation of the ct-DNA binding of methylene blue
Mudasira, Wahyuni E.T.a, Tjahjono D.H.b, Yoshioka N.c, Inoue H.c
a Department of Chemistry, Faculty of Mathematics and Natural Sciences, Gadjah Mada University, Sekip Utara, Indonesia
b School of Pharmacy, Bandung Institute of Technology, Indonesia
c Department of Applied Chemistry, Keio University, Japan
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]The ct-DNA binding properties of methylene blue (MB) including binding constant, thermodynamic parameter and thermal denaturation (T m) have been systematically studied by spectrophotometric method. The binding of MB to ct-DNA is quite strong as indicated by remarkable hypochromicity, red shift and equilibrium binding constant (K b). Van’t Hoff plot of 1/T versus ln K b suggests that the MB dye binds exothermically to ct-DNA which is characterized by large negative enthalpy and entropy changes. According to polyelectrolyte theory, the charge release (Z) when ct-DNA interacts with MB is +1.09 which corresponds very well to the one positive charge carried by the MB dye. The K b at a low concentration of salt is dominated by electrostatic interaction (90%) while that at a high concentration of salt is mostly controlled by non-electrostatic process (85%). However, the stabilization of the DNA binding event in both cases is governed by non-electrostatic process. A moderate stabilization of double helix ct-DNA occurs when the MB dye binds to ct-DNA as indicated by the increase in T m of ct-DNA of about 5.5 °C in the presence of MB. This suggests that MB dye possibly binds to ct-DNA via electrostatic and intercalation modes. © 2010 Elsevier B.V.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Binding constant,Cationic dyes,Charge release,DNA binding,DNA-binding properties,Double helix,Electrostatic interactions,Electrostatic process,Entropy changes,Equilibrium binding constant,High concentration,Hypochromicity,Low concentrations,Methylene Blue,Negative enthalpy,Positive charges,Red shift,Spectro-photometric method,Spectroscopic studies,Thermal denaturations,Thermodynamic parameter,Van’t Hoff plots[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Cationic dyes,DNA,Methylene blue,Thermal denaturation,Thermodynamic parameter[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work is partially supported by Directorate General of Higher Education (DGHE), Ministry of National Education, The Republic of Indonesia through Competency Research Grant (Penelitian Hibah Kompetensi) for the fiscal year of 2008–2009. The partial support from Japan Student Services Organization (JASSO)-Japan through Follow-up Research Fellowship to the first author (M) is also gratefully acknowledged.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/j.saa.2010.06.032[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]