[vc_empty_space][vc_empty_space]
A simple method for screening antihyperlipidemic agents
Hasimun P.a, Sukandar E.Y.a, Adnyana I.K.a, Tjahjono D.H.a
a School of Pharmacy, Institute of Technology Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Screening of antihyperlipidemic agents generally takes 1 -2 month using hyperlipidemia-animal model induced by high cholesterol diet. The aim of this research was to develop a method for screening of antihyperlipidemic agents in relatively short time. This method takes only 8 days, yet it gives similar result compared to conventional method. Animals were orally induced with propylthiouracil of 10 mg kg-1 b.wt. dosage and 0.01% PTU in drinking water for 7 days. On day 8 test drugs were given to animals orally. One hour after test drugs administration, animals were given a solution of high dosage cholesterol in vegetable oil of 400 mg kg-1 b.wt. Serum total cholesterol level was measured in every 1 h after administration of cholesterol for 6 h. After 6 h, a level of total cholesterol in the liver and feces were measured. Result showed that administration of cholesterol in hypothyroid animals significantly raised the serum total cholesterol level in 6 h compared to normal animals induced by cholesterol without previous PTU administration. Administration of low dose of propylthiouracil and 0.01% PTU in drinking water for 7 days before the cholesterol played importance role in increasing the serum total cholesterol level in blood. This method is simpler and requires less time to get hyperlipidemia animal model. Serial measurement of serum total cholesterol level in every hour for 6 h gave a cholesterol profile that can explain different drug mechanisms in cholesterol homeostasis. © 2011 Asian Network for Scientific Information.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antihyperlipidemic agents,Cholesterol,Propylthiouracil,Screening,Total cholesterol level[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.3923/ijp.2011.74.78[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]