[vc_empty_space][vc_empty_space]
Variants analysis of human mitochondrial genome mutation: Study on Indonesian human tissues
Ngili Y.a,b, Noer A.S.a, Ahmad A.S.a, Syukriani Y.F., Natalia D.a, Syah Y.M.a
a Institut Teknologi Bandung, Indonesia
b Department of Chemistry, Universitas Cenderawasih, Indonesia
c Department of Forensic Medicine and Medicolegal, Hasan Sadikin Hospital, Bandung/Faculty of Medicine, Universitas Padjadjaran, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Comparison of variants of mutations that occur in the Indonesia human mitochondrial genome between tissues which origins are from layers of endoderm, mesoderm, and ectoderm have been investigated. In this study, analyzed the diversity of the mtG in various tissues of different origins, namely layer of entoderm, mesoderm, and ectoderm are compared with sequence of revised Cambridge Reference Sequence (rCRS). Determination of mtDNA mutations carried by the isolation procedure followed the protocol of DNA purification from tissues, amplification by using the technique repli-G, determining the nucleotide sequence of the mtG Sanger dideoxy method, and mtDNA in silico sequence analysis. Here we showed that mutation variants between tissues in a normal human individual (not associated with diseases caused by mtDNA mutations) are monomorphic. The results of mutation analysis of mtG variants to rCRS showed that nucleotide mutations in several regions, and found eight variants of the novel mutations that have not been reported in MITOMAP.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]mtDNA mutation,mtG,repli-G,Variants[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]