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Over production of human recombinant interferon α-2b: Scaling up process
Susilo R.A.a, Sumirtapura Y.C.a, Rachmawati H.a, Darijanto S.T.a, Retnoningrum D.S.a, Kardono L.B.S.b,c
a School of Pharmacy, Bandung Institute of Technology, Indonesia
b International Center for Interdisciplinary and Advanced Research, Research Center for Chemistry, Indonesian Institute of Science, Indonesia
c Faculty of Pharmacy, University of Pancasila, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]This research aimed to exercise a large scale (bioreactor) process based on a laboratory experiment and to get control of process parameters on a large scale (bioreactor) to prepare an protein interferon α-2b. The study was initiated by a laboratory-scale protein interferon α-2b overproduction, as a preparation for performing a bioreactor process scale-up. Bioreactor scale-up was performed on a scale of 10 L, based on oxygen mass transfer coefficients and k Lα values between laboratory scale and bioreactor scale. To determine the k Lα, an optimization process was carried out considering a variety of important variables affecting the process in a bioreactor scale. Subsequently, the obtained parameters in the form of aeration velocity and process control were selected. Further, an optimization was also by varying the fermentation time at 40, 60, 72, 80 and 96 h, to get the highest yield of substrate and the protein. Our experiment showed that the k Lα (oxygen mass transfer coefficient), incubation time and aeration velocity were the most important parameters influencing the up production process of the protein up to 10x scaling up. Protein purification was performed by using a nickel affinity column chromatography, while purified protein was analyzed by SDS-PAGE. The quality of interferon resulted from bioreactor scale was similar to that of laboratory scale. © 2012 Asian Network for Scientific Information.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bioreactor scale-up,Incubation time,Laboratory experiments,Laboratory scale,Optimization process,Oxygen mass transfer,Process parameters,Process scale-up,Production process,Protein purification,Purified protein,Scale-up,Scaling-up,SDS-PAGE[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bioreactor,Interferon a-2b,Oxygen mass transfer coefficient (k La),Recombinant protein,Scale up process[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.3923/jas.2012.1613.1617[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]