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Cloning and sequence analysis of lipase gene from DMS3 isolate
Widhiastuty M.P.a, Wahyudi S.T.a,b, Moeis M.R.a, Madayanti F.a, Akhmalokaa
a Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia
b Biophysics Division, Department of Physics, Bogor Agricultural University, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Genes encoding a lipase from DMS3, isolated from Kawah Domas hotspring, Indonesia was cloned and characterised. The lipase gene was cloned based on PCR amplification from genomic DNA. Two set of primers was designed to amplify the lipase gene. An open reading frame of 1248 bps encoding polypeptide of 416 amino acid residues, has been amplified and sequenced. The sequence of amplicon showed high homology (99%) with lipase from Geobacillus thermoleovorans. Detail comparison among three highest homology of lipase showed that there are some variation of amino acid residues. However the subtitution of amino acid residue in lipase ITB3.1, especially at amino acid residue 194 from Asp to Asn is predicted to showh no significant different for the characteristic of lipase ITB3.1 compared to that the other lipase from family 1.5.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Geobacillus thermoleovoran,Kawah Domas hot spring,Lipase,PCR cloning[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.13005/bbra/980[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]