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Development of growth protein delivery system in bone implant based bionanocomposite
Mauludin R.a, Suciati T.a, Pratiwi H.a
a School of Pharmacy, Institut Teknologi Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Currently tissue engineering therapy has been developed. In this therapy, bone formation cells were induced by biodegradable matrix (scaffold) and added by bone growth factor. Chitosan as matrix was chosen because of excellent biocompatibility, biodegradability and osteoconductive properties. Unfortunately chitosan has low mechanical strength. Chitosan was combined with hydroxyapatite (HA) to improve mechanical strength. Commercial hydroxyapatite was incorporated into chitosan matrix. The HA dispersion in matrix was extruted using needle size 27 gauge into NaOH solution until microspheres obtained. The microspheres were characterized using SEM and XRD. Dried microspheres were flushed by acetic acid and followed by pouring slurry into mold. Obtained scaffold was evaluated including to density, porosity and mechanical strength. Redispersion commercial hydroxyapatite had particle size average of 472 nm. PXRD analyses revealed that all bionanocomposite has hydroxyapatite. The analyses were characterized by peak at 2θ of 25°. Morphology of the microspheres was analyzed by SEM and showed microsphere formed relative spherical but its surface was rough. Early analyses of protein entrapment showed that 27% protein was successfully loading scaffold. Density evaluation showed a tendency of increasing density by increasing hydroxyapatite content. Porosity was increase due to increasing of microsphere size of scaffold. An optimum mechanical strength was obtained in combination chitosan: hydroxyapatite = 80: 20 with mechanical strength of 24.47 ± 0.45 Mpa. Increasing hydroxyapatite reduced mechanical strength due to hydroxyapatite can inhibit chitosan sintering process.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bionanocomposite,Chitosan,Hydroxyapatite,Mechanical strength,Microspheres,Scaffold[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]