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The analytical application of cloud point extraction: A simple spectrophotometric method forselenium determination in Kangkong (Ipomoea reptans P.)
Oktariza Y.a, Dwichandra Putra O.a, Insanu M.a, Musadad Miftah A.a
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objective: The aim of the study was to develop a simple spectrophotometric method to quantify Selenium in kangkong (Ipomoea reptans P.). Method: The method was based on complex formation between Selenium and dithizone with cloud point extraction using Triton X-100 to simplify the technique. Result: Optimum condition was established with using of 45 μmol L-1for dihizone, 0.4 M for HCl, 0.4% w/v for Triton X-100, and 15 minutes for incubation time. Under optimum condition, the response was linear between 10 and 110 ppb with correlation coefficient 0.998 and in all cases relative standard deviation was less than 5%. Limit of detection and limit of quantification was found 6.634 and 20.104 ppb respectively. The method was successfully applied to determination of Selenium with recovery 92.833 %. Conclusion: A simple spectrophotometric method has been developed using dithizone as chromogenic agent and cloud point extraction to simplify the technique. The analytical performance full filled the requirement for validation method.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Dithizone,Kangkong and Spectrophometric,Selenium[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]