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Antioxidant capacities from different polarities extracts of cucurbitaceae leaves using FRAP, DPPH assays and correlation with phenolic, flavonoid, carotenoid content
Fidrianny I.a, Darmawati A.a, Sukrasnoa
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objectives: The objectives of this research were to study antioxidant capacity from different polarities extracts of Cucurbitaceae leaves using two methods of antioxidant testing which were DPPH (2.2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total phenolic, flavonoid and carotenoid content in different polarities extracts of Cucurbitaceae leaves with DPPH and FRAP antioxidant capacities. Methods: Extraction was performed by reflux using different polarities solvents. The extracts were vaporated using rotavapor. Chromatogram pattern on each extracts were observed by thin layer chromatography (TLC). Then antioxidant capacities were tested using DPPH and FRAP assays. Determination of total phenolic, flavonoid and carotenoid content were performed by spectrophotometry UV-visible and its correlation with FRAP and DPPH antioxidant capacities were analyzed by Pearson method. Results: LA3 (ethanolic extract of Luffa acutangula leaves) had the highest DPPH scavenging capacity with IC50 73 ppm, while SE2 (ethyl acetate extract of Sechium edule leaves) had the highest FRAP capacity with EC50 759 ppm. SE2 contained the highest total phenolic (4.01 g GAE/100 g), MC1 (n-hexane extract of Momordica charantia leaves) had highest flavonoid content (14.37 g QE/100 g) and highest carotenoid 19.53 g BET/100 g. Conclusions: There were positively high correlation between total phenolic content in Sechium edule leaves extracts with their antioxidant activity using FRAP and DPPH assays. FRAP capacities in Sechium edule leaves extracts had positively high correlation with their DPPH scavenging capacities.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antioxidants,Carotenoid,Cucurbitaceae leaves,DPPH,Flavonoid,FRAP,Phenolic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]