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Transesterification of Linoleic acid in grape seed [Vitis vinifera l.]oiland its analytical method development using gas chromatography
Damayanti S.a, Sigi V.A.a, Julianti E.a
a Pharmacochemistry Research Group, School of Pharmacy-Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objective: Linoleic acid is an essential fatty acid that humans need to ingest. It is the main compound of grape seed [Vitis vinifera L.] oil which is known as inhibitor of hyperproduction of melanin pigment. The objective of this research is to obtain volatile transesterified linoleic acid and to develop its analytical method. Methods: Sample A was obtained by soxhletation with n-hexane at 60°C and sample B was commercial grape seed oil obtained mechanically by expeller pressure. Linoleic acid methyl ester was obtained by acid pretreatment with sulfuric acid-methanol and base transesterification with sodium hydroxide-methanol. Results: The optimized condition was resulted at an isothermal column temperature at 190°C for 12 minute whereas the injector and detector were kept at each 250°C and 280°C.Linearity was confirmed over a calibration range with regression coefficients [r] of 0.999 and coefficient variance of linear regression [Vx0] of 1.670%. The limit of detection [LOD] and limit of quantitation [LOQ] of this method were determined from the coefficient of variation of a known concentration of reference standards resulting 38.480 ppm and 116.620 ppm, respectively. Precision test revealed a coefficent of variance of 1.910% for the first day, 0.850% for the second day, and 1.980% for the third day. Accuracy revealed a percentage of recovery in a range of 98.510-99.830 %. Conclusion: Linoleic acid in grape seed [Vitis vinifera L.] oil was succesfully transesterified and its analytical method development using gas chromatography was validated. Concentration of linoleic acid in sample A and B were 60.233 and 70.393%, respectively.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Gas Chromatography,Grape seed oil,Linoleic acid,Transesterification,Validation of Analytical method[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]