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Bacterial diversity in subbituminous coal and soil from coal mine of South Sumatra, Indonesia
Pikoli M.R.a,b, Aditiawati P.a, Astuti D.I.a, Akhmalokaa
a Microbial Biotechnology Research Group, School of Life Science and Technology, Institut Teknologi Bandung, Indonesia
b Department of Biology, State Islamic Universi ty Syarif Hidayatullah Jakarta, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Coal and soil around coal seam environment provide potential factors that support bacterial life. Study of environmental metagenome to determine relationship among the bacteria that make up the community in coal and soil layers is reported. DNA extractions were performed in three different methods, namely direct extraction, filtration, and filtration with blending. The DNA of coal and soil samples were subject to PCRamplification to get V5-V6 16S rRNA gene fragments, then separated by denaturing gradient gel electrophoresis (DGGE). Results showed that the indirect methods are advantageous and can detect more bands compared to the direct method. Among the samples tested, a high number of bacterial ribotypes (Shannon diversity index), but low evenness of the bacterial community were observed in the coal samples. Cluster analysis of DGGE bands showed that the coal mixed soil clustered separate from its parent, the coal seam and the soil samples. Phylogenetic trees of their sequences showed that the coal boomed more Firmicutes and Actinobacteria compared to the soil samples. It confirmed that the physico-chemical properties of soil strongly influence evolutionary distance of coal and soil bacteria though they are separated by little physical distance. This is the first report in which the disturbed coal which is represented by coal mixed soil was not artificially reconstructed as a microcosm, and is in a natural situation in which the virgin coal seam is compared to the adjacent virgin soil layers and soil mixed coal as the disturbed soil. © OmicsVista Group, All rights reserved.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]16S rRNA,Biodiversity,Coal,PCR-DGGE,Soil[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]