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Increase of oxidative stress and accumulation of α-synuclein in Wistar rat’s midbrain treated with rotenone
Yulianti A.B.a, Sumarsono S.H.b, Ridwan A.b, Yusuf A.T.b
a Medical Biology, Bandung Islamic University, Indonesia
b Research Group of Physiology, Developmental Biology and Biomedical Science, School of Life Science and Technology, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]A neurodegenerative disorder caused by oxidative stress leads to an accumulation of α-synuclein on neurons. Here, we report our findings on the effect of rotenone on the increase of oxidative stress and accumulation of α-synuclein in the midbrain of Wistar rats. Thirty six male rats (8-9 weeks, 200-250 g) were divided into three groups: the Blank group, the Solvent-rotenone group (intraperitoneally injected with 1 ml/kg body weight of sunflower seed oil), and the Rotenone group (intraperitoneally injected with 2.5 mg/kg body weight of rotenone) for 9, 19, and 28 days. The rats were decapitated on day 10 for the 9-day treatment, day 20 for the 19-day treatment, day 30, 40, 50 and 60 for the 28-day treatment (2 rats/group or 6 rats/day of observation time). The midbrains were isolated and extracted. Glutathione assay and α-synuclein ELISA tests were performed. The results showed that the average oxidative stress index was highest in the Blank group (0.95 ± 0.24, 0.63 ± 0.23, 0.81 ± 0.27, respectively). Meanwhile, the concentration of α-Synuclein had decreased in all groups (0.09 ± 0.03, 0.15 ± 0.03, and 0.13 ± 0.02 ng/mg tissue, respectively). Correlation analysis showed that the oxidative stress index was inversely proportional to the concentration of α-synuclein. Our conclusion is that the midbrain of Wistar rats treated with rotenone indicated oxidative stress and led to an accumulation of α-synuclein protein. © 2012 Published by LPPM ITB.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antioxidant,Neurodegeneration,Oxidative stress,Rotenone,α-synuclein[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.5614/itbj.sci.2012.44.4.3[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]