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Isolation, identification of phenylacetic acid from Streptomyces galbusTP2 strain and its toxicity
Sri Andayani D.G.a, Sukandar E.Y.a, Sukandar U.a, Ketut Adnyana I.a
a Chemical Engineering, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Streptomyces, which constitutes one genus of Actinomycetes class usually found in the soil, has specific characteristics that can be utilized as a producer of antibiotics. The Objective of this research wasto find out the safety of compounds resulting from the broth of StreptomycesgalbusTP2 strain on test animals and normal cells. The fermentation liquid from Streptomyces galbusTP2 strainwas extracted by ethyl acetate, then purified through vacuum liquid chromatography and radial chromatography. The identification of pure compound was done bya UV spectrophotometer, (1H, 13C, Dept, HMBC and HMQC) NMR, and LCMS. The result of structural elucidation showed that the pure molecule was Phenylacetic acid. Theacute toxicity test was LD50> 15 g/kg of body weight(subsequently abbreviated into bw) against mice and cytotoxic test was IC50 =1228 μg/ml against the normal cell, this indicating that it was safe to be a raw material medicine.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Acute Toxicity,Cytotoxic,Streptomyces sp.,Structural Elucidation[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]