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Development of jacalin conjugated nanostructured lipid carriers formula for transcutaneous vaccine delivery
Suciati T.a, Samhana A.a, Mauluddin R.a
a School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Objective: Most of transcutaneous vaccines consist of protein or peptide antigens having poor immunogenicity, high molecular weight, and hydrophilic properties. These features become the major challenge in developing a non-invasive vehicle for targeting to skin antigen presenting cells (APCs). This research aimed to develop a transcutaneous vaccine formula in the form of nanostrucured lipid carriers (NLCs). Methods: NLCs were prepared by emulsification and solidification method. Bovine serum albumin (BSA) was used as antigen protein model, which was incorporated into lipophilic phase as solid dispersion form in PEG 20000. NLCs were constructed from a mixture of Suppocire® NA and oleic acid as lipophilic phase and stabilized by Lutrol® the F 68 as surfactant and ethanol as cosurfactant. Jacalin, a lectin isolated from jackfruit (Artocarpus integrifolia) seed used as an adjuvant, was conjugated on the surface of BSA-loaded NLCs under acidic condition by utilizing electrostatic interaction. Results: The average particle size of the resulting dispersion was 382.3±10.80 nm with a polydispersity index of 0.159±0.02 and zeta potential of -2.96±0.71 mV. BSA entrapment efficiency in NLCs was higher than 80%. TEM observation revealed the NLCs spherical particles with un-aggregated BSA dispersed homogenously. Hemagglutination test indicated that jacalin was adsorbed onto the surface of BSA-loaded NLCs as active form. The physical stability evaluations including determination of average particle size, size distribution, and zeta potential showed relatively stable nanoparticles during 4-week storage. Conclusion: The NLCs obtained having high payload of protein and surface anchored jacalin is promising as a transcutaneous vaccine platform.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Adjuvant,Co-lyophilization,Jacalin,Microemulsion,NLCs,Protein,Transcutaneous vaccine[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]