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Antioxidant activities, Total flavonoid, Phenolic, Carotenoid of various shells extracts from four species of legumes

Fidrianny I.a, Puspitasari N.a, Marlia Singgih W.a

a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia

[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2014 Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.Objectives: The objectives of this research were to study antioxidant capacity from various extracts of legumes shells using two methods of antioxidant testing, which were 2,2-diphenyl-1 picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total flavonoid, phenolic, and carotenoid content in various extracts of legumes shells with DPPH and FRAP antioxidant capacities. Materials and Methods: Extraction was performed by reflux using different polarity solvents. The extracts were vaporated using rotavapor. Antioxidant capacities using DPPH and FRAP assays, determination of total phenolic, flavonoid, and carotenoid content were performed by spectrophotometry UV-visible and its correlation with DPPH and FRAP antioxidant capacities was analyzed by Pearson method. Results: TNH2 (ethyl acetate shells extract of peanut) had the highest DPPH scavenging capacity with IC50 0.595 mg/mL, while MRH2 (ethyl acetate shells extract of red kidney bean) had the highest FRAP capacity with EC50 294.781 mg/mL. TNH2 contained the highest total flavonoid (13.37 g quercetin equivalents/100 g), TNH3 (ethanolic shells extract of peanut) had the highest phenolic content (6.91 g gallic acid equivalents/100 g), and KDL2 (ethyl acetate shells extract of soybean) had the highest carotenoid 0.33 g beta-carotene equivalent/100 g. Conclusions: There were positively and high correlation between total phenolic and total flavonoid content in Bogor peanut and peanut shells extracts with their antioxidant activity using DPPH assays. FRAP capacities in all of the shells extracts had no linier result with DPPH scavenging capacities.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]2,2-diphenyl-1 picrylhydrazyl,Antioxidants,Carotenoid,Ferric reducing antioxidant power,Flavonoid,Legumes Shells,Phenolic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]