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Induction of somatic embryos from leaf and stem nodal section explants of potato (Solanum tuberosum L.)
a Biology Department, School of Life Sciences and Technology, Institut Teknologi Bandung Jl, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2014 Published by ITB Journal Publisher.Somatic embryogenesis can be used as an alternative method to obtain high-quality potato seedlings. This research was conducted in order to evaluate: the potential of different types of explants to produce somatic embryos; combinations of 2,4-D and BAP for somatic embryo induction; and the ability of BAP to act as growth regulator for the somatic embryo maturation process. Young leaf and stem nodal sections were cultured in MS media supplemented with 2,4-D and BAP. The developing calli were then transferred into media containing several concentrations of 2,4-D and BAP. The embryogenic calli were then transferred into media containing 1-5 μM BAP. The results show that MS media containing 2,5 μM 2,4-D + 5 μM BAP and μM 2,4-D + 0.5 μM BAP were the most suitable for inducing somatic embryos from leaf explants and stem nodal section explants, respectively. The somatic embryos were well-developed in the MS media supplemented with 5 μM BAP. The stem nodal section explants were able to produce a higher percentage of globular, heart and torpedo stage embryos compared with the leaf explants. Based on this research, stem nodal section explants have a high potential for producing somatic embryos induced in medium containing 1 μM 2,4-D + 0.5 μM BAP.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]2,4-D,BAP,Potatoes (Solanum tuberosum L.),Somatic embryogenesis,Stem node,Tissue culture,Young leaf[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.5614/j.math.fund.sci.2014.46.3.8[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]