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Method development for simultaneous analysis of marker scopoletine, andrographolide, quercetin, and luteolin in antihypertension jamu formulation using RP-HPLC
Fidrianny I.a, Rukoyah U.a, Komar Ruslan W.a
a School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, International Journal of Pharmacy and Pharmaceutical Science. All rights reserved.Objective: The aim of this research was to develop a method of simultaneous analysis marker compounds in antihypertension jamu formulation using reverse phase high performance liquid chromatography (RP-HPLC). Methods: Stationary phase, mobile phase, sample preparation and instrument condition were optimized. Results: Analysis of marker compounds in anti hypertension jamu formulation using HPLC with reverse phase ODS C18 column (LiChroCART®, 125 x 4.6 mm, particle size of 5 μm) and gradient mobile phase of formic acid 1% and methanol could separate markers in 35 min at flow rate 2 mL/min. Four markers in antihypertension jamu formulation have been successfully analyzed simultaneously, were scopoletine, andrographolide, quercetin, and luteolin. The RSD values demonstrated the intra and inter-day precisions were less than 4% and 6.5% respectively and the recoveries around 93.45-114.42%, respectively. All calibration curves showed good linear correlation coefficient which of R > 0.995. Conclusion: RP-HPLC method has been developed for simultaneous analysis of four markers in antihypertension jamu. The methods can be used to analyze antihypertension jamu formulation in the market.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Andrographolide,Antihypertension jamu formulation,HPLC,Luteolin,Quercetin,Scopoletine[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]