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Estimation of the potential of an anaerobic thermophilic oil-degrading bacterium as a candidate for MEOR
Purwasena I.A.a, Sugai Y.b, Sasaki K.b
a School of Life Science and Technology, Bandung Institute of Technology, Bandung, 40132, Indonesia
b Department of Earth Resources Engineering, Faculty of Engineering, Kyushu University, Fukuoka, 819-0395, Japan
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2013, The Author(s).We investigated the decrease in oil viscosity caused by the biodegradation of crude oil by a bacterium (AR80) isolated from an oil reservoir, and estimated the potential for this bacterium for use in microbial enhanced oil recovery (MEOR). AR80 degraded long-chain n-alkanes preferentially, and anaerobically increased the ratio between the short-chain and long-chain n-alkane concentrations in the crude oil. The long-chain n-alkane metabolism by AR80 decreased the oil viscosity. AR80 grew well in a reservoir of brine supplemented with yeast extract, and decreased the oil viscosity to approximately 60 % of its original value. Adding a small amount of yeast extract (0.05 g/L) was necessary to stimulate the AR80 activity. MEOR can, therefore, be achieved using AR80 without incurring excessive costs. AR80 can grow at temperatures up to 80 °C and grows well at between 50 and 70 °C. AR80 can grow at a salinity of up to 90 g/L and grows well at a salinity of <30 g/L. The AR80 activity was not affected very much by high pressures (such as 6.0 MPa). Core flooding experiments were performed by injecting AR80 (in brine supplemented with yeast extract) into Berea sandstone cores. Gas chromatography analysis of the effluent oil suggested that long-chain n-alkanes in the residual oil were preferentially degraded by the AR80 in the porous rocks. The core flooding experiments showed that the AR80 activity in the porous rocks caused the oil recovery to be enhanced, so AR80 could be a suitable candidate for MEOR.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Author keywords" size="size-sm" text_align="text-left"][vc_column_text]Core flooding,Microbial enhanced oil recoveries,Oil viscosity,Oil-degrading bacteria,Reservoir conditions,Yeast extracts[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Indexed keywords" size="size-sm" text_align="text-left"][vc_column_text]Core flooding,Microbial enhanced oil recovery,Oil viscosity,Oil-degrading bacteria,Reservoir conditions,Yeast extract[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Funding details" size="size-sm" text_align="text-left"][vc_column_text]This work was supported by JSPS KAKENHI Grant Number 21760678 and Kyushu University G-COE program Novel Carbon Resource Science. We gratefully acknowledge the INPEX Corporation in Japan for their support and cooperation in providing the brine sample. We are also grateful to Dr. Kazuhiro Fujiwara for his technical advice. We appreciate technical support for our anaerobic incubation experiments provided by the Research Support Center, Graduate School of Medical Sciences, Kyushu University.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="DOI" size="size-sm" text_align="text-left"][vc_column_text]https://doi.org/10.1007/s13202-013-0095-5[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]