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Chemical composition and antimicrobial activity of diterpene and essential oils of hedychium roxburghii blume rhizome
a Department of Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.Objective: The objective of the present study was to isolate and determine diterpene compound and essential oils from Hedychium roxburghii Blume rhizome and investigated those antimicrobial activities. Methods: The essential oils were obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by gas chromatography-mass spectrometry (GC/MS) method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-nuclear magnetic resonance (NMR), 13C-NMR, heteronuclear single quantum coherence-NMR, heteronuclear multiple bond correlation-NMR and carbon coupling 13C-NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method. Results: The oils exhibited antimicrobial activity against Bacillus subtilis American Type Culture Collection (ATCC) 6633 (minimum inhibitory concentration [MIC] 1750 μg/ml), Staphylococcus aureus ATCC 6538 (MIC 1750 μg/ml), Escherichia coli ATCC 8939 (MIC 3500 μg/ml), Pseudomonas aeruginosa ATCC 9027 (>3500 μg/ml) and Candida albicans ATCC 10231 (MIC 875 μg/ml). A phytochemical study of the rhizome essential oils of H. roxburghii Blume were performed by GC/MS and the result showed that fenchyl acetate (45.85%) was the main component of the oils. Compound 1 was identified as diterpene compound, coronarin E. Coronarin E have not exhibited MIC at 512 μg/ml, however, it showed inhibition profile against all of tested microbes. Conclusion: The essential oils and ethanolic residual-distillation extract of H. roxburghii Blume rhizome exhibited weak antimicrobial profile. Compound 1 was identified as diterpene compound, (coronarin E), it was exhibited weak antimicrobial activity, but showed inhibition profile against all of the tested microbes.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antimicrobial,Coronarin E,Essential oils,Hedychium roxburghii,Zingiberaceae[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]