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Antioxidant activities of various extracts from ardisia sp leaves using dpph and cuprac assays and correlation with total flavonoid, phenolic, carotenoid content
Fidrianny I.a, Budiana W.b, Ruslan K.a
a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
b Bandung School of Pharmacy, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, International Journal of Pharmacognosy and Phytochemical Research. All Rights Reserved.The objectives of this research were to study antioxidant activity from various leaves extracts of four Ardisia sp using two methods of antioxidant testing which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPRAC (cupric ion reducing antioxidant capacity) and correlation of total flavonoid, phenolic and carotenoid content in various leaves extracts of four Ardisia sp with IC50 of DPPH and EC50 of CUPRAC antioxidant capacities. Extraction was performed by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and CUPRAC assays, determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with IC50 of DPPH scavenging activities and EC50 of CUPRAC capacities were analyzed by Pearson’s method. All of sample extract was categorized as very strong antioxidant. Ethanolic leaves extract of A.crenata (CR3) had the lowest IC50 of DPPH scavenging activity with IC50 0.49 mg/ml and ethyl acetate leaves extract of A. ellipticagave the lowest EC50 of CUPRAC capacity with EC50 30.34 mg/ml. Ethyl acetate leaves extract of A. cymosa (CY2) contained the highest total flavonoid (8.24 g QE/100 g), ethanolic leaves extract of A.elliptica(EL3) showed the highest phenolic content (29.54 g GAE/100 g) and n-hexane leaves extract of A.fuliginosa (FU1) had the highest total carotenoid 13.37 g BE/100 g. There were negatively high correlation between total phenolic content in A.crenata, A.cymosa and A.fuliginosaleaves extracts with their IC50 of DPPH scavenging activities. There were negative and high correlation between total flavonoid in all of sample extract and EC50 of CUPRAC capacities. Total carotenoid content in A.elliptica, A.cymosa andA.fuliginosaleaves extracts had negative and high correlation with their CUPRAC capacities. A.crenataleaves extracts had linear result in DPPH and CUPRAC assays.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antioxidant,Ardisia sp,Carotenoid,CUPRAC,DPPH,Flavonoid,Leaves,Phenolic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]