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Antihyperuricemic activity of four plants annonaceae using hyperuricemic rats model and enzyme assay
Sunarni T.a, Leviana F.a, Fidrianny I.b, Iwo M.I.b, Wirasutisna K.R.b
a Department of Pharmaceutical Biology, Setia Budi University, 57127, Indonesia
b Pharmaceutical Biology Research Group, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.Objective: The aim of the research is to study the antihyperuricemic activity of Annona muricata L, Annona squamosa L, Annona reticulata L, and Stelechocarpus burahol (Bl.) Hook. F. & Th. (Annonaceae). Methods: The dried powdered leaves were extracted using ethanol by maceration method. The ethanolic extracts of four plants were administered orally to potassium oxonate-induced rats. Serum samples were collected from rats at the 1stto 3rd hr after drug administration. Enzyme assay was done by xanthine oxidase (XO). The XO inhibitory activity was measured by UV-Vis spectrophotometry, and the degree of enzyme inhibition was determined by measuring the absorbance of uric acid formation. Results: The result of this study revealed that all of the ethanolic extracts had a significant effect on hyperuricemic rats, and S. burahol extract decreased uric acid level was equal to level normal control. However, the antihyperuricemic activity was not parallel with XO inhibitor activity which showed weak activity with IC50 of A. reticulata was 171.73 μg/ml, while IC50 of A. muricata, A. squamosa, and S. burahol more than 200 μg/ml. Conclusion: The ethanolic extract of S. burahol was more potential than three other extracts in decreasing in uric acid levels. The results scientifically confirmed that S. burahol can be developed as potential antihyperuricemic agents.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Annona muricata,Annona reticulata,Annona squamosa,Antihyperuricemia,Potassium oxonate,Stelechocarpus burahol,Xanthine oxidase[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]