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In vitro antioxidant capacities of three organs of bitter gourd (Momordica charantia L.) form west java-indonesia using DPPH and FRAP assays
Fidrianny I.a, Susilawati R.D.a, Ruslan K.a
a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reservedThe objectives of this research were to study antioxidant capacity from various organs extracts of bitter gourd using two methods of antioxidant testing which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) and correlation of total flavonoid, phenolic and carotenoid content in various organs extracts of bitter gourd with IC50 of DPPH and IC50of FRAP antioxidant capacities. Extraction was performed by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant capacities using DPPH and FRAP assays, determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with IC50 of DPPH scavenging capacities and IC50of FRAP capacities were analyzed by Pearson’s method. All of extracts sample (except n-hexane pedicel extract, n-hexane fruit extract and ethyl acetate fruit extract) were categorized as strong and very strong antioxidant by DPPH method. Phenolic compounds were the major contributor in antioxidant activities of various organs (leaves, pedicel and fruit) extracts of bitter gourd by DPPH and FRAP assays. All of organs extracts of bitter gourd had linear result in DPPH and FRAP assays.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Antioxidant,Bitter gourd,DPPH,FRAP,Organs[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]