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Antihyperuricemia activity of the ethanol extract of roselle calyx and its fraction (Hibiscus sabdariffa Linn) on male wistar rats
Wahyuningsih S.a,b, Sukandar E.Y.a, Sukrasnoa, Lofika D.N.b
a School of Pharmacy, Bandung Institute of Tecnology, Bandung, Indonesia
b Universitas Jenderal Achmad Yani, Jalan Terusan Jenderal Sudirman, Cimahi, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016 The Authors.Objective: The objective of this study was to determine anti-hyperuricemia activity of ethanol extract of roselle calyx (Hibiscus sabdariffa L) and its fraction on male Wistar rats. Methods: Two different methods were used. First method induction was done by giving potassium oxonate and diet high purine whereas allopurinol as a comparison. The parameter was level uric acid in serum (uricostatic activity). Second methods induction was done by giving potassium oxonate intraperitoneally and sodium urate orally; with pprobenecid as comparison Parameters used were uric acid level in serum and urine (uricosuric activity). Results: Ethanol extract roselle calyx at a dose of 40 mg/kg bw, water fraction at a dose of 37.5 mg/kg bw and ethyl acetate fraction at a dose of 6.25 mg/kg bw at day 14 showed a significant reduction in serum uric acid level compared to control group (p<0.05). Besides that ethanol extract of Roselle calyx at dose 80 mg/kg bw, water fraction at doses 37.5 and 75 mg/kg bw also ethyl acetate at doses 6.25 and 12.5 mg/kg bw had uricosuric effect that increased the excretion of uric acid in urine significantly compared to control group (p<0.05). Conclusion: The water fraction of roselle calyx at doses 37.5 and 75 mg/kg bw and ethyl acetate at dose 6.25 and 12.5 mg/kg bw was effective as an anti-hyperuricemic agent. The ethanol extract of rosele calyx and its fraction had a uricosuric effect similar to probenecid. The best uricosuric effect was showed by ethyl acetate fraction at dose 6.25 mg/kg bw.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Author keywords" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Indexed keywords" size="size-sm" text_align="text-left"][vc_column_text]Antihyperuricemia,Calyx,Ethyl acetate fraction,Hibiscus sabdariffa linn,Uric acid,Water fraction[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Funding details" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="DOI" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width="1/4"][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]