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In vitro antioxidant activities in various beans extracts of five legumes from west of java-indonesia using DPPH and ABTS methods
Fidrianny I.a, Elviana D.a, Ruslan K.a
a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.The aim of this research were to determine antioxidant activity from different polarities beans extracts of five legumes beans using two methods of antioxidant testing which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) and correlation of total phenolic, flavonoid and carotenoid content in various extracts of five legumes beans with their IC50 of DPPH and IC50 of ABTS antioxidant activities. Extraction was done by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and ABTS assays, determination of total phenolic, flavonoid and carotenoid content were conducted by UV-visible spectrophotometry and its correlation with IC50 of DPPH and IC50 of ABTS scavenging activities were analyzed by Pearson’s method. All of ethanol extracts from five legumes beans were categorized as very strong antioxidant by DPPH and ABTS methods. Phenolic compounds in red kidney bean extracts were the major contributor in IC50 of DPPH and IC50 of ABTS scavenging activities. DPPH and ABTS assays showed linear results in red kidney bean and bogor peanut sample.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]ABTS,Antioxidant,Carotenoid,DPPH,Flavonoid,Legumes beans,Phenolic[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]