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Evaluation of antioxidant activities of fruit extracts of chayote (Sechium edule [Jacq.] Swartz) grown in different sites in java-Indonesia
Fidrianny I.a, Kevina, Hartati R.a
a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.Objectives: The aim of this research were to determine antioxidant activity from various fruit extracts of chayote from three different sites using two antioxidant methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phosphomolybdenum methods, correlation of total phenolic, flavonoid, and carotenoid content in various extracts of chayote with their IC50of DPPH antioxidant activities and EC50of phosphomolybdenum capacity. Methods: An extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by ultraviolet-visible spectrophotometry and its correlation with IC50of DPPH and EC50of phosphomolybdenum were analyzed by Pearson’s method. Results: The lowest IC50of DPPH scavenging activity was given by n-hexane fruit extract of chayote from Lembang (9.32 μg/ml), while the lowest EC50of phosphomolybdenum capacity was given by ethyl acetate fruit extract of chayote from Semarang (209.87 μg/ml). Ethyl acetate chayote fruit extract from Malang gave the highest phenolic content and its n-hexane extract had the highest total flavonoid. There were negative and significant correlation between total flavonoid content in all of the chayote fruit extracts from three different sites with their IC50of DPPH and EC50of phosphomolybdenum. Conclusions: N-hexane chayote fruit extract from Lembang and Semarang and ethyl acetate chayote fruit extract from Semarang were categorized as a very strong antioxidant by DPPH method. Flavonoid compounds in all of the chayote fruit extracts from three different locations were the major contributor in their antioxidant activities by DPPH and phosphomolybdenum methods. All of the chayote fruit extracts from Lembang, Semarang, and Malang had a linear result in DPPH and phosphomolybdenum assays.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]2,2-diphenyl-1-picrylhydrazyl,Antioxidant,Chayote,Different sites,Fruit,Phosphomolybdenum[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]