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Antioxidant activities from various leaves extracts of three cultivars of papaya from west Java-Indonesia
Fidrianny I.a, Paramitha K.a, Kusmardiyani S.a
a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.Objectives: The goals of this research were to determine antioxidant activity from various leaves extracts of three cultivars of papaya using two methods of antioxidant testing which were 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and correlation of total phenolic content (TPC), total flavonoid content (TFC), and total carotenoid content (TCC) in various extracts of papaya leaves with their IC50of DPPH and IC50of ABTS. Methods: Extraction was carried out by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and ABTS assays, determination of TPC, TFC, and TCC were performed by ultraviolet-visible spectrophotometry and correlation with their IC50of DPPH and IC50of ABTS scavenging activities were analyzed by Pearson’s method. Results: The lowest IC50of DPPH scavenging activity 0.84 μg/ml was given by ethanolic leaves extract of calina papaya, whereas ethanolic leaves extract of Bangkok papaya showed the lowest IC50of ABTS scavenging activity 1.79 μg/ml. Ethanolic leaves extract of burung papaya had the highest phenolic content and its ethyl acetate extract had the highest total flavonoid. There were significantly negative correlation between TPC in Bangkok papaya and calina papaya extract with their IC50of DPPH. Conclusions: All of the leaves extracts from three cultivars of papaya were categorized as very strong antioxidant by DPPH and ABTS methods. Phenolic compounds in Bangkok papaya and calina papaya were the major contributor in IC50of DPPH scavenging activities. DPPH and ABTS gave no linear result in antioxidant activities of leaves extract of three cultivars of papaya.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]2,2-diphenyl-1-picrylhydrazyl,2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid),Antioxidant,Leaves,Papaya,Three cultivars[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]