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Anti-inflammatory study of Anredera cordifolia leaves and Centella asiatica herbs and its combinations using human red blood cell-membrane stabilization method
Sutrisno E.a,b, Ketut Adnyana I.a, Sukandar E.Y.a, Fidrianny I.a, Aligita W.b
a Department of Pharmacology, Bandung Institute of Technology, Bandung, Indonesia
b Department of Clinical and Community Pharmacy, Bandung School of Pharmacy, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.Objective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was the highest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system. This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica). Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human red blood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin and asiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug. Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit the hemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combination of ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition of hemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm. Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or as combinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Anredera cordifolia,Anti-inflammation,Centella asiatica,Human red blood cell – membrane stabilization[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.22159/ajpcr.2016.v9i5.11973[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]