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Antioxidant activities from various extracts of different parts of kelakai (Stenochlaena palustris) grown in central Kalimantan – Indonesia
Kusmardiyani S.a, Novita G.a, Fidrianny I.a
a Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.Objectives: The aims of this research were to determine antioxidant activity from various extracts of different parts of kelakai (Stenochlaena palustris [Burm.f.] Bedd) using two antioxidant testing methods, which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP), and correlation of total phenolic contents (TPC), total flavonoid contents (TFC), and total carotenoid contents (TCC) with their inhibitory concentration 50% (IC50) of DPPH and exhibitory concentration 50% (EC50) of FRAP. Methods: Sample was extracted by reflux using different polarity solvents. The extracts were evaporated using vacuum rotary evaporator. Antioxidant activities were tested using DPPH and FRAP assays, determination of TPC, TFC, and TCC was carried out by ultraviolet-visible spectrophotometry, and correlation with their IC50 of DPPH and EC50 of FRAP capacities was analyzed by Pearson’s method. Results: Ethanolic root extract of kelakai (S. palustris) had the lowest IC50 of DPPH scavenging activity 0.8 µg/ml and the lowest EC50 of FRAP capacity 5.4 µg/ml. Ethanolic kelakai root extract demonstrated the highest phenolic content, ethyl acetate young leaves extract had the highest flavonoid content, and the highest carotenoid content was given by n-hexane root extract. There was significantly negative correlation between TPC in root extract of kelakai with their IC50 of DPPH and EC50 of FRAP. Conclusions: All different extracts of kelakai parts were categorized as very strong antioxidants by DPPH method. Phenolic compounds in kelakai root extract were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant activities of root kelakai extract.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]2-diphenyl-1-picrylhydrazyl,Antioxidant,Ferric reducing antioxidant power,Old leaves,Root,Stenochlaena palustris,Young leaves[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.22159/ajpcr.2016.v9s2.13630[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]