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Effects of low dose aspirin on caspase 3, TNF-α and apoptotic index levels in preclampsia maternal serum-induced placental trophoblast cell line in vitro
Pramatirta A.Y.a, Laksono B.a, Fauziah P.N.b, Anwar A.D.a, Krisnadi S.R.a, Retnoningrum D.S.c, Maskoen A.M.a, Widiarsih E.a
a Department of Obstetrics and Gynecology, Faculty of Medicine, Padjadjaran University, Indonesia
b Department of Medical Laboratory Technology, School of Health Sciences Jenderal Achmad Yani Cimahi, Indonesia
c School of Pharmacy, Bandung Institute of Technology, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2016, Sphinx Knowledge House. All rights reserved.Preeclampsia is a major cause in maternal mortality and morbidity. Placental apoptosis is found to be increased in preeclampsia and it is associated with caspase-8 activation on extrinsic pathway, followed by caspase-3 activation which is responsible as executioner caspaseto generate apoptosis. Trophoblasts express tumor necrosis factor (TNF) that contain a cytoplasmic “death domain’’ that mediates apoptotic signals. Low dose aspirin is widely used in preventing preeclampsia reducing apoptosis induced by H2O,and reduce ability of caspase-3 activation.This study aims to examine the difference between apoptotic index, TNF-α and caspase-3expression in trophoblast cells induced by normal and preeclampsia maternal serum, and effects of aspirin onapoptotic index, TNF-α and caspase-3 in preeclampsia and normal-induced trophoblast. Methods: This was experimental study in laboratory. Apoptotic index was measured byAnnexin V-Fluous Stainingand expressionof TNF-α and caspase-3 were performed by ELISA. Results: It was found that expression of TNF-α and caspase-3,and apoptotic index in preeclampsia serum-induced trophoblast cells were higher than normal and controls. Low dose aspirin of0,5 mM showed significant decreased expression of TNF-α, caspase-3 andapoptotic index (p<0.05) which equal normal and controls. Conclusion: Apototic index, expression of TNF-αand caspase-3 are affected by serum and aspirin dosage.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Author keywords" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Indexed keywords" size="size-sm" text_align="text-left"][vc_column_text]Apoptotic index,Aspirin,Caspase 3,Preeclampsia,TNF-α,Trophoblast[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Funding details" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="DOI" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width="1/4"][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]