Bioprospecting of Turbinaria Macroalgae as a Potential Source of Health Protective Compounds
Stranska-Zachariasova M.a, Kurniatanty I.b, Gbelcova H.a, Jiru M.a, Rubert J.a, Nindhia T.G.T.c, D’Acunto C.W.a, Sumarsono S.H.b, Tan M.I.b, Hajslova J.a, Ruml T.a
a Department of Food Analysis and Nutrition, University of Chemistry and Technology, Prague, Prague, 166 28, Czech Republic
b School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40116, Indonesia
c Department of Mechanical Engineering, Engineering Faculty, Udayana University, Jimbaran, Bali, 80361, Indonesia
Abstract
© 2017 The Authors Chemistry & Biodiversity Published by Wiley-VHCA AGThe present study aims to focus on the bioprospecting of marine macroalgae of Turbinaria species, plenteous biomass of the world ocean. Three types of solvents, i.e., H2O, MeOH/H2O (80:20, v/v) and hexane/i-PrOH (50:50, v/v), were used for extraction. Both the biological activity and the pattern of present chemicals were characterized. For the cell proliferation assay, the human embryonic kidney 293 cells, cervix/breast/pancreatic adenocarcinoma, and osteosarcoma cells were used. For the antioxidant activity determination, both intracellular assay with human embryonic kidney and cervix adenocarcinoma cells, as well as the biochemical DPPH test, were employed. To complete the information about macroalgae composition, organic compounds were characterized by the liquid chromatography coupled with high resolution tandem mass spectrometry. Attention was concentrated mainly on the lipidomic profile characterization. In spite the fact that any significant antiproliferative effect was not observed for cancer cells, both the Turbinaria species were shown to be good protectors against the oxidative stress of the non-cancer cells. Most of the antioxidants were determined in the hexane/i-PrOH extract. As regards the lipids identified, most of them belonged to the triacylglycerols followed by sphingomyelins, diacylglycerols, and polar (lyso)phospholipids. Additionally to fatty acids with 14, 16 and 18 carbons, also those with odd carbon numbers were frequently present.
Author keywords
Antioxidants,Biological Products,Bioprospecting,Cell Proliferation,Cells, Cultured,Chromatography, High Pressure Liquid,Humans,Lipids,Metabolome,Metabolomics,Microalgae,Protective Agents,Tandem Mass Spectrometry
Indexed keywords
Algae,Antioxidant activities,Antiproliferation assay,Bioprospecting,Liquid chromatography,Mass spectrometry,Turbinaria
Funding details
This work has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 692195. The partial financial support was also received from the Operational Programme Prague ? Competitiveness (CZ.2.16/3.1.00/21537 and CZ.2.16/3.1.00/24503) and by the ?National Program of Sustainability I? – NPU I (LO1601 – No.: MSMT-43760/2015). Authors are also grateful to the Ministry of Education, Youth and Sport of the Czech Republic, for the project CZ.1.07/2.3.00/30.0060 supported by the European Social Fund. Partial funding was also received by Ministry of Religion Affair, Republic of Indonesia (No.: 2603/2013), and Directorate General of Higher Education of the Ministry Education and Culture, the Republic of Indonesia through Scheme of International Research Collaboration (Penelitian Kerjasama Luar Negri) under contract number: 238-40/UN14.2/PNI.01.03.00/2014 granted through Udayana University.