ARID1A expression is down-regulated by oxidative stress in endometriosis and endometriosis-associated ovarian cancer
Winarto H.a, Tan M.I.b, Sadikin M.a, Wanandi S.I.a
a Gynecologic Oncology Division, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
b School of Life Sciences and Technology, Bandung Institute of Technology, Bandung, Indonesia
Abstract
© The Author(s) 2017.Oxidative stress is considered an important factor in the development of endometriosis, including its malignant transformation. Previous studies have found that AT-rich interactive domain 1A (ARID1A), a tumor suppressor gene, is frequently mutated and inactivated in endometriosis-associated ovarian cancer (EAOC), and such a change in this gene is considered an early event in malignant transformation. We observed oxidative stress status by measuring the activity of the antioxidant enzyme manganese superoxide dismutase (MnSOD), malondialdehyde (MDA), and ARID1A gene expression in tissue samples from patients with endometriosis, EAOC, or non–endometriosisassociated ovarian cancer (non-EAOC). We also induced oxidative stress in the cultured cells from patients with primary endometriosis by adding H2O2 and tested for any alteration of ARID1A gene expression based on different H2O2 concentrations. The results showed that MnSOD activity in endometriosis and EAOC was lower than in non-EAOC, but MDA levels were higher. This study also showed that oxidative stress reduced ARID1A gene expression.
Author keywords
Indexed keywords
ARID1A,Endometriosis,Malignant transformation,Ovarian cancer,Oxidative stress,Tumor suppressor gene