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Co-delivery of curcumin-loaded nanoemulsion and Phaleria macrocarpa extract to NIH 3T3 cell for antifibrosis
Rachmawati H.a, Novel M.A.a, Nisa R.M.a, Berlian G.b, Tandrasasmita O.M.b, Rahma A.a, Riani C.a, Tjandrawinata R.R.b
a School of Pharmacy, Bandung Institute of Technology, Bandung, 40132, Indonesia
b Dexa Laboratories of Biomolecular Sciences, Cikarang – Jababeka, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2017 Elsevier B.V.Antifibrotic effect of curcumin-loaded nanoemulsion and its combination with Phaleria macrocarpa extract (PM) was determined through evaluation on collagen expression in fibroblast NIH/3T3 cells. Prior to activity study, cytotoxic effect of blank nanoemulsion in vitro was determined on different cell types using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The genes expression of collagens were determined with quantitative real time PCR. The amount of collagen protein was determined using Sirius Red/Fast Green Collagen Staining Kit. Stability of curcumin-loaded nanoemulsion towards hepatic metabolism was evaluated as well. Cells viability was preserved after treatment with curcumin- and curcumin-PM nanoemulsion. Both preparations decreased procollagen α1(1) and collagen IV genes and collagen protein. Nanoemulsion demonstrated protective effect against hepatic metabolism of curcumin. Curcumin nanoemulsion and curcumin-PM nanoemulsion showed a promising antifibrotic agent.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Collagen,Curcumin,Liver fibrosis,Nanoemulsion,Phaleria macrocarpa,Procollagen α1(1)[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/j.jddst.2017.03.015[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]