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Isolation, characterization and identification of lipolytic thermophiles with methanol tolerance from domestic compost
Syihab S.F.a, Madayanti F.a, Akhmalokaa
a Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2015, Journal of Pure and Applied Microbiology. All rights reserved.Five thermophilic bacteria with lipolytic activity were isolated from domestic compost namely AL17, AL59, AL64, AL89, and AL96. All of the isolates showed rod shape and gram negative microbes. The lipase activity among the isolates are varies when grown in medium with pH 7.0 and pH 9.0. AL17 and AL89 isolates showed best activity in medium with pH 7.0. Meanwhile AL64 and AL96 showed less activity in both type of the media, in addition AL59 was found to be mostly active when cultivated in pH 9.0. Methanol tolerant properties of the isolates was investigated through addition of 3% methanol in medium. Growth rate measurement of the isolates showed that AL96 was better methanol tolerant properties compared to others. The 16S rRNAs genes of all five bacteria have been sequenced and deposited to GenBank. Phylogenetic analysis of the 16S rRNA genes showed that four isolates close to genus Pseudoxanthomonas, while the other was highly similar to Brevibacillus thermoruber.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]16S rRNA,Lipase,Methanol tolerant bacteria[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work was funded by ITB-Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia through Decentralization Research Programme.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]