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Development of molecular imprinted polymer solid phase extraction for separation nitrofurantoin residue in chicken eggs
Damayanti S.a, Gunawan U.a, Ibrahim S.a
a Department of Pharmacochemistry, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2017 The Authors.Background: The use of nitrofurantoin and other nitrofuran antibiotics in food which produced from animals is prohibited by the European Union because of potentially carcinogenic and mutagenic. Various methods for analysis of residues of nitrofurantoin have been developed, but because of the interference of the matrix, it is necessary to separate the matrix therefore, the matrix effect will not interfere the analysis. Nowadays, molecular imprinted polymer (MIP) is a well-developed tool in the analytical field, mainly for separating substances in relatively complex matrices. Objective: The purpose of this study is to obtain molecular imprinted solid phase extraction (MISPE) that is selective for the separation of nitrofurantoin residues in chicken eggs. Methods: Analytical methods development of nitrofurantoin was optimization of high-performance liquid chromatography (HPLC) system and validation of analytical methods performed to obtain the suitable system for nitrofurantoin detection. In silico study used for MIP design by observing the difference Gibbs free energy using Gaussview 5.08 software with density functional theory methods using 6-311 G as basis set. MIP synthesis was done using bulk method use nitrofurantoin as template, acrylamide as functional monomer, ethylene glycol dimethacrylate as crosslinker, and azobisisobutyronitrile as an initiator reaction inside dimethylformamide (DMF) as a solvent. Non-imprinted polymer (NIP) was synthesized as a comparison. MIP and NIP which has been synthesized was inserted into solid phase extraction (SPE) cartridge and characterized using infrared spectroscopy and HPLC. Result: MISPE that has been synthesized was characterized and compared to NIPsolid phase extraction (NISPE) and marketed SPE C18. The sensitivity of MIP, NIP, and SPE C18 to nitrofurantoin was 84.54%, 37.73%, and 33.95%, respectively, based on recovery of nitrofurantoin. Conclusion: Based on the result it was obtained MISPE has high selectivity toward nitrofurantoin compared to NISPE and either marketed SPE.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]High-performance liquid chromatography,In silico,Molecular imprinted solid phase extraction,Non-imprinted solid phase extraction,Solid phase extraction C18[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors gratefully acknowledge financial support by Ministry of Finance Indonesia through Indonesia Endowment Fund for Education (LPDP).[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.22159/ajpcr.2017.v10i6.17109[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]