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Evaluation of the enzymatic hydrolysis process of Oil Palm empty fruit bunch using crude fungal xylanase
Mardawati E.a, Purwadi R.b, Kresnowati M.T.A.P.b, Setiadi T.b
a Department of Food Technology, Universitas Padjadjaran, Jatinangor, Indonesia
b Department of Chemical Engineering, Institut Teknologi Bandung, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2006-2017 Asian Research Publishing Network (ARPN).Oil Palm empty fruit bunches (OPEFB) is the solid waste produced from crude palm oil industries. It comprises of cellulose, hemicellulose and lignin. The major component of hemicelluloses, xylan, is a complex heteropolysaccharide with a β-1,4-xylose backbone. In the utilization of xylan, it must first be hydrolyzed into its components, that are the 5 carbon atom sugar xylose and arabinose. Endoxylanase (xylanase) is the primary enzyme that attacks the backbone structure of hemicellulose by random cleavage of internal xylosidic linkages to produces the xylose hydrolisate. Further xylose can be used as raw material for the production of a wide variety of chemicals such as xylitol. Enzymatic hydrolysis of xylan to xylose using xylanase offers an environmentally friendly biotechnological process beside performed at ambient temperature and pressure, it also has high specificity and low cost. Previous research has shown that fungal species Trichoderma is a good producer of lignocellulosic enzymes including xylanase. This article describes the study of enzymatic hydrolysis of OPEFB using crude xylanase extract produced by Trichoderma viride ITB CC L67. The study foccused on the optimation of hydrolysis process in terms of temperature and pH. The optimization was done based on Response Surface Methodology (RSM) to give either the optimum xylose concentration or xylose to glucose concentration ratio. Further, the kinetics of the enzymatic hydrolysis process of OPEFB was studied. The enzymatic hydrolysis can be well approached by the Michaelis Menten kinetic model, and kinetic parameters were obtained from experimental data.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Enzymatic hydrolysis,Oil palm empty fruit bunches,Optimization,Xylanase and xylose[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]