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Determining the enzyme accessibility of ammonia pretreated lignocellulosic substrates by Simon’s Stain method
Octavia S.a, Purwadi R.b, Arsa I.D.G.P.b, Soerawidjaja T.H.b
a Department of Chemical Engineering, Universitas Bung Hatta, Padang, Indonesia
b Department of Chemical Engineering, Bandung Institute of Technology, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2006-2017 Asian Research Publishing Network (ARPN).The promising technology to assess the susceptibility of ammonia-treated of lignocellulosic biomass potential to enzymatic hydrolysis in bioconversion process to biofuels is Simon’s Stain measurement. Fundamentally, the porosity and the overall surface area of lignocellulosic substrate will successfully increase by the pretreatment process; as the major structural feature influencing the hydrolysis of pretreated substrates by cellulases. The modified of Simon’s Stain method was used in this study by decreasing the processing time from > 50 to 6 hours, is the semi quantitative method for estimating the available surface area of ammonia-treated OPEFB and SSB. The materials, oil palm empty fruit bunches (OPEFB) and sweet sorghum bagasse (SSB), were soaked into aqueous ammonia for 1, 2 and 7 days in room condition. The data shown, the maximum dye adsorbed on the ammonia-treated materials was increasing as raise the pretreated-days as well. The total dye adsorption correlated well with the enzymatic hydrolysis yields resulting in good correlation coefficient (R2). This method proved to be an effective tool for assessing the potential of cellulase to hydrolyze the lignocellulosic substrate.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Adsorption,Cellulase,OPEFB,Simon’s stain method,Soaking with aqueous ammonia,SSB[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]