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Estimating numbers of oil palm (elaeis guineensis) pollen grains using image analysis and processing
Permana A.D.a, Permana B.a, Sahari B.b, Putra R.E.a, Kinasih I.c
a Institut Teknologi Bandung, School of Life Sciences and Technology, Jalan Ganesha, Bandung, West Java, 40132, Indonesia
b Research and Development, Plant Protection Laboratory, PT Astra Agro Lestari Tbk, Kumai, Indonesia
c Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Gunung Djati, Bandung, West Java, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Elaeidobius kamerunicus is the most important oil palm pollinator in Indonesia and Malaysia. However, the mechanism and efficiency of pollen transfer by this weevil are clearly not understood. The lack of study on pollination process in oil palm (Elaeis guineensis) is mostly caused by difficulties in pollen counting due to their small size. Most of the counting was conducted manually which is prone to mistakes, required extensive training, and time-consuming. The aim of this study is to provide a novel technique for counting pollen that is rapid, consistent, and efficient with a comparable accuracy to manual counting. Male and female of E. kamerunicus were collected from male and female oil palm inflorescences (N=60). Extracted pollen were placed and distributed in a flat microscope slide separated by designated observation chambers. Images of each chamber were captured as a JPEG format and analysed by ImageJ. Multiple macros were constructed for image processing steps to obtain the pollen numbers. Comparison with manual counting using paired T-Test, Pearson’s correlation and linear regression showed a high similarity between both methods.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Elaeidobius kamerunicus,Elaeis guineensis,Image processing,Imagej,Pollen counting[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]We thank Palgunadi and Satyoso for allowing us to use the laboratory and photographic equipment; Agus Widodo, Bargowo Addianto for allowing us to use their personnel; Ali Imran and Jari for helping with manual counting; Prima for her assistance in the field; Erwin for helping with statistical analysis; Ageng Mufti SH for support and critical suggestion in developing macro; Damiran and Hani for helping with research material preparation; Firman, Yusuf, Solihul Amal, Ramadhani Eka, M Kamal Muzzaki, Adeline Prapaisie, Yuvika, Ni Putu Indah, Wahyu Santika for valuable suggestion during research design. This research was partly funded by PT Astra Agro Lestari Tbk and PUPT Grant from Ministry[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.21894/jopr.2017.2903.02[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]