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Phylogeny of Indonesian durian (Durio sp.) germplasm based on polymorphism of ITS-nrDNA sequences
Santoso P.J.a, Indriyani N.L.P.a, Istianto M.a, Pancoro A.b, Aryantha I.N.P.b
a Indonesian Tropical Fruit Research Institute, Indonesian Agency for Agricultural Research and Development Solok, West Sumatra, Indonesia
b School of Life Science and Technology, Institut Teknologi Bandung, Bandung, West Java, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]Indonesia is considered as the center of origin of durian, and has abundant species and cultivars. Phylogenetic analysis is one of the methods to study the genetic structure of germplasm for management purposes. This research aimed to analyze the molecular phylogeny of Indonesia’s durian germplasm based on polymorphism of conserved ITS-nrDNA region. One hundred and forty-nine durian germplasm samples were used in this study. ITS sequences of the germplasm samples and those from GenBank were aligned using Clustal W, and a phylogenetic tree was reconstructed using Geneious R6 PC software. The results demonstrated that the ITS-nrDNA region is highly conserved in genus Durio and is a powerful tool to determine durian systematics at the interspecies level. The phylogenetic tree could be drawn with speciation of several doubtful accessions and new emerging durian variations could be confirmed. The tree conveyed low evolutionary resolution of the region around Durio zibethinus; therefore, this gene is not suitable for identification of genetic diversity of durian at the intraspecies level in this species.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Durian,Germplasm management,ITS,Phylogeny,Speciation[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors thank all donors of samples used in the study, especially Dr M. Reza Tirtawinata for pictures of ‘Sigundul’ and ‘Sipakem’. This research was funded by the KKP3T Program of IAARD, contract number 965/LB.620/I.1/3/2011.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.17660/ActaHortic.2017.1186.5[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]