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Trypsin inhibitor activity and protein analysis of gall rust from sengon plants (Falcataria moluccana Miq.) infected with Uromycladium tepperianum fungus
a School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2018 Alfi Rumidatul et al.Background and Objectives: Sengon Falcataria moluccana (F. moluccana) has the ability to synthesize protease inhibitors, such as trypsin inhibitors, that can inhibit the activity of an enzyme produced by Uromycladium tepperianum (U. tepperianum), a fungus that causes gall rust disease. The purpose of this research is to study the activity of the trypsin inhibitor, the total protein content and the protein profile in healthy and gall rust infected sengon. Materials and Methods: The samples used in this research were taken from sengon twigs consisting of healthy and gall rust infected bark and wood. In this research, three age classes of sengon were used: 1-2 years, 3-4 years and 5-6 years. The method used in this research was field sampling, trypsin inhibitor activity analysis, protein determination and protein profile analysis by SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Results: The results showed that, in general the activity of trypsin inhibitors in the gall rust infected sengon twigs was higher than in the healthy sengon twigs. The highest protein content was found in gall rust infected bark twigs and the lowest protein content was in healthy wood twigs. The results of the SDS-PAGE analysis of healthy and gall rust infected bark showed that there was a protein with a molecular weight of 21 kDa, which was identified as a trypsin inhibitor. Conclusion: Falcataria moluccana infected with U. tepperianum fungus showed an increase in the activity of trypsin inhibitors and protein content.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Gall rust disease,Protein,SDS-PAGE,Sengon,Trypsin inhibitors[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]Acknowledgments are addressed to the Ministry of Research, Technology and Higher Education Indonesia as the sponsor for this research with project grant number 009/SP2H/LT/DRPM/IV/2017 and the mycology laboratory of the Center for Research on Biosciences and Biotechnology, ITB for providing facilities and support to this research.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.3923/ajps.2018.75.84[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]