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Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis
Widowati W.a, Laksmitawati D.R.b, Wargasetia T.L.a, Afifah E.c, Amalia A.c, Arinta Y.c, Rizal R.c, Suciati T.d
a Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia
b Faculty of Pharmacy, Pancasila University, Jakarta Selatan, 12630, Indonesia
c Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung, 40163, Indonesia
d School of Pharmacy Bandung Insitute of Technology, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2018, Mashhad University of Medical Sciences. All rights reserved.Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)). Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2Htetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucoseinduced mesangial cells were assayed and determined using ELISA KIT. Results: In viability assay, MPE 5 and 20 μg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) (P<0.05). In inhibitory activity of TGF-β1 and fibronectin level, MPE 5 μg/ml (glucose-induced 5 mM) show the lowest level compared to positive control and other treatments (P<0.05). Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β1 and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Author keywords" size="size-sm" text_align="text-left"][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Indexed keywords" size="size-sm" text_align="text-left"][vc_column_text]Factor-β1,Fibronectin,Garcinia mangostana,Glomerulosclerosis,Mesangial cell,Transforming growth[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="Funding details" size="size-sm" text_align="text-left"][vc_column_text]This research was funded and facilitated by Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia. The author also thankful to Hanna Sari W Kusuma, Rismawati Laila Qodariah, Fajar Sukma Perdana, Annisa Arlisyah, Ni Luh Wisma Ekayanti from Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia for their valuable assistance.[/vc_column_text][vc_empty_space][vc_separator css=".vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}"][vc_empty_space][megatron_heading title="DOI" size="size-sm" text_align="text-left"][vc_column_text]https://doi.org/10.22038/ijbms.2018.29349.7094[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]