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Production of biopolymer polyhydroxyalkanoates (PHA) by extreme halophilic marine archaea Haloferax mediterranei in medium with varying phosphorus concentration
Melanie S.a, Winterburn J.B.b, Devianto H.c
a Research and Development Center for Marine and Fisheries Product Processing and Biotechnology, Ministry of Marine Affairs and Fisheries, Jakarta, 10260, Indonesia
b School of Chemical Engineering and Analytical Science, The University of Manchester, M13 9PL, United Kingdom
c Department of Chemical Engineering, Faculty of Industrial Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]©2018 Published by ITB Journal Publisher.The development of plastics production from biodegradable resources such as polyhydroxyalkanoates (PHAs) is important due to the increasing demand for plastics. PHAs occur as intracellular solid materials produced by microorganisms as a result of an excess of carbon source and a limitation of nutrients. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) or PHBV, as one of the PHAs, is a combination of two homopolymers, namely poly-3-hydroxybutyrates (PHB) and poly-3-hydroxyvalerates (PHV). This study aimed to investigate the effect of the phosphorus concentration on the production of PHAs by Haloferax mediterranei. It is possible that phosphorus deficiency may affect the length of the HV chains in the copolymer structure and thus influence the copolymer properties. The experiment was done in triplicate at laboratory scale by culturing H. mediterranei in medium with phosphorus limitation using various phosphorus concentrations. During cultivation, the optical density, phosphorus concentration, pH, and dry cell weight were observed. The PHBV product was collected and analyzed using gas chromatography. The result shows that medium with a phosphorus concentration of 0.5 g/L produced higher PHAs than the other phosphorus concentrations. The accumulated PHA was 0.95 g/L with 15.6% of dry biomass and yield YPHA/S of 0.1 g/g.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bioplastics,Haloferax mediterranei,PHBV,Phosphorus limitation,Poly-hydroxyalkanoate[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bioplastic,Biopolymer,Haloferax mediterranei,PHA,PHBV,Phosphorus limitation,Polyhydroxyalkanoate[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]We thank the Indonesia Endowment Fund for Education (Lembaga Pengelola Dana Pendidikan, LPDP) within the Ministry of Finance, Republic of Indonesia, for funding this research. We also thank Anna Ferre Guell for giving support and guidance in the laboratory and analysis. In addition, we also would like to thank Fernando Guzman Lagunes for his helpful discussion.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.5614/2Fj.eng.technol.sci.2017.50.2.7[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]