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The response of TLR3 and IL-1β genes following exposure to LPS, Poly (I:C), zymosan in culture of gouramy (osphronemus gouramy) kidney cells
Kusumawaty D.a, Suhandono S.b, Aryantha I.N.P.b, Pancoro A.b
a Department of Biology Education, Indonesia University of Education, Bandung, 40154, Indonesia
b School of Life Sciences and Technology, Bandung Institute of 14 Technology, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2018 Universiti Putra Malaysia Press.The aims of the present study are to isolate and characterise structure TLR3 and IL1β gene and evaluate the potential and signaling mechanism following exposure of Polyinosinic: Polycytidylic acid (poly (I:C)), Lipopolysaccharide (LPS) and Zymosan as antigens in gouramy (Osphronemus gouramy). Gouramy kidney cells were stimulated with LPS, Poly I:C and Zymosan. Following incubation at 28°C, relative expression levels of Toll Like Receptor 3 (TLR3) and interleukin-1β (IL-1β) were examined at one hour and six hour after treatment. A Real Time Polimerase Chain Reaction approach was utilised to search for the effects of Poly I:C, LPS, and Zymosan exposure to gouramy kidney cells between one hour to six hours after treatment, LPS in kidney cell increased expression of interleukin-1β and downregulated expression of TLR3. Poly (I:C) which is an antigen that responds to antiviral, induces an increase in the transcription both of Toll like receptor 3 and IL-1β. Zymosan in kidney cells increased expression of TLR3 but downregulated expression of IL1β. This study shows that TLR3 was activated not only by Poly I: C but also LPS and Zymosan. However, antigen Poly IC-induced labor IL-1β and TLR3 expressed higher than the antigen LPS and Zymosan, so, this research showed a TLR3 response to Poly I:C is more dominant than Zymosan and LPS. TLR3 in gouramy kidney cell transcript was identified in response to poly I:C, Zymosan, and LPS. These results indicate that TLR3 in gouramy also plays a role for defense against bacterial infection and virus.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]IL1β,Innate immune,LPS,Osphronemus gouramy,Poly(IC),TLR3,Zymosan[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to extend their gratitude to the support received from Doctoral Scholarship Programme by DIKTI (Directorate General of Higher Education Indonesia) and to Professor Ikuo Hirono, from Laboratory of Genome Science, Tokyo University of Marine Science and Technology, for providing LPS, Poly IC and Zymosan.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]