[vc_empty_space][vc_empty_space]
The use of response surface method in optimization of levan production by heterologous expressed levansucrase from halophilic bacteria bacillus licheniformis BK2
Permatasari N.U.a,b, Ratnaningsih E.a, Hertadi R.a
a Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia
b Chemistry Departement, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2018 Published under licence by IOP Publishing Ltd.Response surface method (RSM) is a recent common method used to identify culture condition for optimal production of particular metabolite. In the present study, RSM is used to optimize a catalytic reaction in levan production by heterologous expression of lsbl-bk2 gene isolated from halophilic bacteria Bacillus licheniformis. Levan is a polyfructose polymer produced from sucrose by the action of extracellular levansucrase secreted by the microorganism. Three factors for levan production, namely sucrose concentration, pH, and temperature of reaction were optimized by full factorial and central composite designs in RSM. The result indicated that the optimum in vitro condition for levan production was achieved when the levansucrase catalytic reaction was performed at 32?C, pH 8, in 12% (w/v) sucrose solution. Levan produced by this procedure was verified by FTIR and NMR spectroscopies.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Bacillus licheniformis,Catalytic reactions,Central composite designs,FTIR and NMR spectroscopy,Halophilic bacteria,Heterologous expression,Response surface method,Sucrose concentration[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work is funded and supported by Doctoral Dissertation Research Grant form Indonesian Ministry of Research and Technology with the contract number of 123/SP2H/PTNBH/DPRM 2018 and P3MI.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1088/1755-1315/209/1/012015[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]