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A novel acemannan-chitosan modified lipid nanoparticles as intracellular delivery vehicles of antibiotic
Suciati T.a, Rachmawati P.a, Soraya E.a, Mahardhika A.B.a, Satrialdia, Hartarti R.a, Anggadiredja K.a
a Department of Pharmaceutics, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2018 Tri Suciati et al.Killing intracellular pathogens is often hampered by poor antibiotic penetration. A nanoparticle formulation with surface properties similar to microbial particles can be advantageous as a carrier for intracellular delivery of antimicrobial agents. Surface-modified lipid nanoparticles with chitosan-conjugated acemannan-an acetylated polymannose of aloe gel isolate, have been proposed to deliver rifampicin intracellularly. Chitosan-acemannan (COSACE) conjugate was bound electrostatically to rifampicin-loaded nanostructured lipid carrier (NLC) to form COSACE lipid nanoparticles. The nanoparticles showed a diameter size of around 300 nm at a low polydispersity index, and positive zeta potential of 1.66 ± 1.39 mV. Transmission electron microscope and confocal images showed that the COS-ACE lipid nanoparticles were spherical in shape with NLC in the center surrounded by COS-ACE conjugate. Rifampicin was highly entrapped within lipid nanoparticles and released from the particles at pH 5 at higher rate compared with pH 7.4. The lyophilized nanoparticles were stable at the storage temperature of 4°C and 25°C, in terms of the content and particle size. Furthermore, the COS-ACE lipid nanoparticles was non-toxic against Vero and BALB/c 3T3 cells and increased intracellular accumulation of lipid soluble antibiotic, suggesting its functionality in intracellular targeting.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Acemannanchitosan conjugate,Intracellular infection,Lipid nanoparticles,Rifampicin[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This research was funded by “Desentralisasi” research grant, The Ministry of Research Technology and Higher Education of the Republic of Indonesia. Chito-olygosaccharide ?COS) used in this work was produced using research grant of Development of Raw Materials for API/excipient and Traditional Medicines, Directorate General of Pharmaceutical and Medical Devices, The Ministry of Health of the Republic of Indonesia. We are grateful to Ms. Fatma Ayatiliulil Albab and Dewi Satwika from PT. Fajar Mas Murni for their assistance in confocal microscope imaging.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.7324/JAPS.2018.81201[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]