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[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019, Society for Indonesian Biodiversity. All rights reserved.Ralstonia solanacearum is a phytopathogenic bacterium causing bacterial wilt disease which has been reported to infect many important plants in Indonesia. However, less study has been done on its genetic diversity in Indonesia. This study aimed to investigate the genetic diversity of R. solanacearum virulent strains isolated from Java Island. BOX-PCR fingerprint was carried out to investigate the genetic pattern of R. solanacearum isolates, while phylogenetic analysis of mutS gene was performed to determine the genetic diversity of R. solanacearum isolates. A total of 21 isolates was obtained from four crop species: twenty isolates identified as phylotype I while one isolate identified as phylotype II. Based on BOX-PCR, most of the isolates clustered according to their original provinces, indicating site-dependent distribution pattern. However, BOX-PCR also detected site-contaminations indicated by the similar genetic patterns found in two provinces. Phylogenetic analysis of mutS gene discovered that most of the phylotype I isolate showed 100% similarity to each other, while phylotype II isolate belonged to phylotype IIB and showed 100% similarity to IPO1609 strain. Pathogenicity and biovar test were confirmed that the phylotype IIB isolate belonged to the R3bv2 strain which was adapted in low temperature. This study provided the first description about genetic diversity patterns of R. solanacearum strains in Java Island and revealed new challenges related to how to prevent contamination of R. solanacearum from one province to another, as well as the phylotype IIB strain infection in a highland area in Indonesia.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]BOX-PCR,Genetic diversity,MutS gene,Ralstonia solanacearum[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to thank PT East West Seed Indonesia for funding our study as well as the staffs from the Laboratory of Plant Pathology PT East West Seed Indonesia for their assistance in collecting samples from infected plants and doing cross-testing. The authors also would like to thank the United States Agency for International Development (USAID) for training and mentoring support in writing this article, through the Sustainable Higher Education Research Alliance (SHERA) Program for Universitas Indonesia’s Scientific Modeling, Application, Research, and Training for City-centered Innovation and Technology (SMART CITY) Project, and Jessica Kahler from University of Florida as our mentor for her willingness to give early review on this paper.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.13057/biodiv/d200209[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]