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Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination
Hamdani S.a,b, Asstiyani N.a, Astriany D.a, Singgih M.b, Ibrahim S.b
a Sekolah Tinggi Farmasi Indonesia, Bandung, Indonesia
b School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019 Institute of Physics Publishing. All rights reserved.Proteolytic bacteria were found in many environment, some were found in soil or mud. This research was conducted to find proteolytic bacteria from pig sludge that is place for pig wallowing. Bacteria isolation was carried out by streak plate method on nutrient agar media. Qualitative test of proteolytic bacteria used skim milk agar medium. The results of isolation obtained 10 bacterias and 2 of them have potential as proteolytic bacteria. Identification of proteolitic bacteria used the 16s rRNA method, result showed that bacterias are Bacillus pseudomycoides and Staphylococcus sciuri. Protease isolation was carried out under 4°C and under room temperature during centrifugation as separation process. Protease activity from Bacillus pseudomycoides was at 4°C as much as 0.094 U/mL and was 0.082 U/mL at room temperature, while from Staphylococcus sciuri was at 4°C as much as 0.120 U/mL and was 0.098 U/mL at room temperature.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Agar media,Agar medium,Bacteria isolation,Isolation and identification,Protease activities,Qualitative test,Separation process,Staphylococcus sciuri[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1088/1755-1315/230/1/012095[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]