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Production of protein hydrolysate containing antioxidant activity from Hermetia illucens
a School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019 The AuthorsProtein hydrolysate is a complex mixture of peptide and amino acids that can be produced from various biomass sources including insects, such as black soldier fly larvae (Hermetia illicens) due to its relatively high protein content. This study aimed to investigate the potential of protein hydrolysate from black soldier fly larvae as a bioactive hydrolysate through enzymatic hydrolysis using bromelain. Black soldier fly larvae contain 25.6% protein and 35.5% lipids as determined by a proximate analysis. Experiments for the enzymatic hydrolysis of black soldier fly larvae was designed using a central composite design with three factors particularly enzyme concentration (1–5%), pH (6–8) and time of hydrolysis (3–24 hours). The protein hydrolysate had a yield of 10.70 % (on a weight basis) based on defatted biomass with a productivity of 21 mg/L/batch. The protein concentration varied between 240-310 μg/ml with the degree of hydrolysis varied in the range of 10–43%. The protein hydrolysate had a molecular weight in the range of 14–25 kDa based on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. The amino acid composition of the protein hydrolysate was also determined and mainly consists of lysine (8.0%), leucine (7.7%), and valine (7.2%). The protein hydrolysate may find application as a bioactive hydrolysate with an antioxidant activity of 72.6 in terms of its ability to inhibit free radicals 2,2-diphenyl-2-picryl hydrazyl with IC50 of 0.84%.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Amino acid,Antioxidant activity,Biochemistry,Biotechnology,Black soldier fly larvae,Chemical engineering,Enzymatic hydrolysis,Protein hydrolysate[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]This work was supported by a research grant from the Ministry of Research, Technology, and Higher Education Indonesia ( PN-1-23-2018 ).[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.1016/j.heliyon.2019.e02005[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]