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Shoot multiplication and growth rates of aquilaria malaccensis lamk. Shoot cultures in temporary immersion system (tis)-rita® and bubble column bioreactors
Esyanti R.R.a, Fadholi M.a, Rizki R.M.a, Faizal A.a
a Plant Science and Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, 40132, Indonesia
[vc_row][vc_column][vc_row_inner][vc_column_inner][vc_separator css=”.vc_custom_1624529070653{padding-top: 30px !important;padding-bottom: 30px !important;}”][/vc_column_inner][/vc_row_inner][vc_row_inner layout=”boxed”][vc_column_inner width=”3/4″ css=”.vc_custom_1624695412187{border-right-width: 1px !important;border-right-color: #dddddd !important;border-right-style: solid !important;border-radius: 1px !important;}”][vc_empty_space][megatron_heading title=”Abstract” size=”size-sm” text_align=”text-left”][vc_column_text]© 2019, Pakistan Botanical Society. All rights reserved.Agarwood is the resinous product of several tree species of family Thymelaeaceae such as Aquilaria spp. Unsustainable harvesting has threatened the existence of agarwood producing species in their natural habitat. Therefore, the present study was developed to facilitate an effective and efficient method to produce Aquilaria malaccensis seedlings by In vitro culture. Here, we optimized the aeration rate in the bubble column reactor and the immersion time on the temporary immersion system (TIS)-RITA® bioreactor and compare the growth rate of A. malaccensis with both systems. A. malaccensis shoot cultures were propagated in Murashige and Skoog (MS) semisolid medium and then pre-conditioned in thin layer culture before bioreactor cultivation. A. malaccensis shoots in the bubble column reactor were subjected to variable aeration rates of 0.05 and 0.1 vvm, while the immersion variation within the TIS-RITA® bioreactor was 5 and 15 min for every 4 h. The results showed that 15 min immersion for every 4 h increased the number of A. malaccensis shoot regeneration. Nonetheless, no significance difference was observed on biomass acquisition in both bubble column bioreactor and TIS-RITA® bioreactor. Therefore, our findings indicate that immersion period was more critical than aeration rate for A. malaccensis shoot cultivation.[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Author keywords” size=”size-sm” text_align=”text-left”][vc_column_text][/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Indexed keywords” size=”size-sm” text_align=”text-left”][vc_column_text]Aquilaria malaccensis,Bubble column bioreactor,Shoot multiplication,TIS-RITA® bioreactor[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”Funding details” size=”size-sm” text_align=”text-left”][vc_column_text]The authors would like to thank Institut Teknologi Bandung for funding this research through P3MI Grant, 2017 (Contract No. 1253/I1.CO2.2/PL/2017).[/vc_column_text][vc_empty_space][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][vc_empty_space][megatron_heading title=”DOI” size=”size-sm” text_align=”text-left”][vc_column_text]https://doi.org/10.30848/PJB2019-4(36)[/vc_column_text][/vc_column_inner][vc_column_inner width=”1/4″][vc_column_text]Widget Plumx[/vc_column_text][/vc_column_inner][/vc_row_inner][/vc_column][/vc_row][vc_row][vc_column][vc_separator css=”.vc_custom_1624528584150{padding-top: 25px !important;padding-bottom: 25px !important;}”][/vc_column][/vc_row]